{"data":{"event":{"id":"d994e778-63fa-49ee-bb49-ec5df40a7148","slug":"rfdiffusion-de-novo-protein-design","title":"RFdiffusion designs binders and assemblies later confirmed by experiment","short_summary":"The Baker lab’s RFdiffusion, published in Nature on 11 July 2023, generated protein backbones whose experimental structures and binding functions, including an influenza HA binder, matched designs.","full_description":"Fine-tuning RoseTTAFold as a diffusion model enabled unconditional monomers, binders, oligomers, and motif scaffolding. Hundreds of designs were characterized; a cryo-EM structure of an HA binder closely matched the design model. Open-source code spread quickly. Human specification of targets and experimental filtering remained essential.","ledger_type":"benefit","primary_domain_id":"8f1af1b9-7302-4b51-aae9-fdfac04d158a","event_status":"verified","event_date":"2023-07-11T00:00:00.000Z","discovery_date":"2023-07-11T00:00:00.000Z","first_published_date":"2023-07-11T00:00:00.000Z","last_reviewed_date":"2023-07-11T00:00:00.000Z","geographic_scope":"Global protein-design community","affected_population":"Academic and industry protein engineers","base_impact_tier":4,"base_score":"30.00","attribution_multiplier":"0.7500","evidence_multiplier":"0.8500","realization_multiplier":"0.6500","durability_multiplier":"0.8500","current_event_score":"8.453300","confidence_level":"high","score_explanation":"General, experimentally validated generative design method with wide uptake (tier 4). Attribution shared with RoseTTAFold and wet lab (0.75). Independent adoption plus cryo-EM (0.85). Realization is a working method used broadly, not approved drugs.","methodology_version_id":"d7881163-fb23-4e71-8625-bac1a8662c0f","original_methodology_version_id":"d7881163-fb23-4e71-8625-bac1a8662c0f","published_at":"2023-07-11T00:00:00.000Z","created_at":"2026-09-13T20:47:20.075Z","updated_at":"2026-09-13T20:47:20.075Z","flags":[],"domain_name":"Biology","domain_slug":"biology","methodology_version":"0.1"},"contributions":[{"id":"a6b4f337-65ba-46fd-b462-82d4ef403b1a","event_id":"d994e778-63fa-49ee-bb49-ec5df40a7148","model_id":"666807a8-da52-45d2-a1ca-a507d12911ce","role_description":"Diffusion model that generated backbones later validated experimentally.","attribution_multiplier":"0.7500","credit_share":"0.8000","contribution_score":"8.453300","attribution_rationale":"Central design engine; sequence design and experiments were additional human/ML steps.","attribution_confidence":"medium","first_used_date":"2023-07-11T00:00:00.000Z","model_version_if_known":null,"review_status":"approved","created_at":"2026-09-13T20:47:20.075Z","model_slug":"rfdiffusion","model_name":"RFdiffusion","identity_class":"named_release","is_internal":false,"family_name":"RoseTTAFold","family_slug":"uw-institute-for-protein-design-rosettafold","organization_name":"University of Washington Institute for Protein Design","organization_slug":"uw-institute-for-protein-design"}],"sources":[{"id":"fb3e9c4e-00ed-4ca0-9725-562e7f27a3a9","event_id":"d994e778-63fa-49ee-bb49-ec5df40a7148","url":"https://www.nature.com/articles/s41586-023-06415-8","canonical_url":"https://www.nature.com/articles/s41586-023-06415-8","source_type":"peer_reviewed","publisher":"Nature","author":null,"publication_date":"2023-07-11T00:00:00.000Z","retrieved_at":"2026-09-13T20:47:20.075Z","title":"De novo design of protein structure and function with RFdiffusion","excerpt":null,"content_hash":null,"source_reliability_class":"high","is_primary_source":true,"is_independent":true,"is_peer_reviewed":true,"archived_url":null,"created_at":"2026-09-13T20:47:20.075Z"},{"id":"37d20387-2a1d-487e-8c4d-543885626c4a","event_id":"d994e778-63fa-49ee-bb49-ec5df40a7148","url":"https://github.com/RosettaCommons/RFdiffusion","canonical_url":"https://github.com/RosettaCommons/RFdiffusion","source_type":"other","publisher":"RosettaCommons","author":null,"publication_date":"2023-07-11T00:00:00.000Z","retrieved_at":"2026-09-13T20:47:20.075Z","title":"RFdiffusion open-source implementation","excerpt":null,"content_hash":null,"source_reliability_class":"low","is_primary_source":true,"is_independent":false,"is_peer_reviewed":false,"archived_url":null,"created_at":"2026-09-13T20:47:20.075Z"}],"claims":[{"id":"c73477c0-0801-40a3-9ecc-19a80c95f685","event_id":"d994e778-63fa-49ee-bb49-ec5df40a7148","claim_text":"RFdiffusion designs were experimentally characterized, including a cryo-EM-confirmed influenza HA binder.","claim_type":"outcome","claim_status":"supported","confidence_score":null,"created_at":"2026-09-13T20:47:20.075Z","updated_at":"2026-09-13T20:47:20.075Z"}],"revisions":[{"id":"50338be4-c01c-43d4-8301-8638037ddccd","event_id":"d994e778-63fa-49ee-bb49-ec5df40a7148","model_id":null,"previous_score":"0.000000","new_score":"8.453300","previous_factors":{},"new_factors":{"evidence":0.85,"base_score":30,"durability":0.85,"attribution":0.75,"realization":0.65},"change_reason":"Initial adjudicated seed score under methodology 0.1.","trigger_type":"seed","trigger_source_ids":null,"reviewer_id":null,"review_status":"published","created_at":"2026-09-13T20:47:20.075Z"}],"secondary":[{"name":"Medicine & Health","slug":"medicine-health"},{"name":"Materials Science","slug":"materials-science"}]},"methodology_version":"0.1"}